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What is used in transcription as a template for the construction of a new rna molecule

What is Used in Transcription as a Template for the Construction of a New RNA Molecule?

In the field of molecular biology, transcription is a crucial process that involves the synthesis of RNA molecules from DNA templates. Understanding the key components involved in this process is essential for grasping the basics of gene expression. This article aims to explain what is used in transcription as a template for the construction of a new RNA molecule, highlighting its benefits and the conditions in which this knowledge is applicable.

Key Points:

  1. DNA as the Template:

    • During transcription, DNA serves as the template for the construction of a new RNA molecule.
    • DNA contains the genetic instructions necessary for the synthesis of proteins.
    • The specific segment of DNA that is transcribed and used as a template is called a gene.
  2. RNA Polymerase:

    • RNA polymerase is the enzyme responsible for catalyzing the process of transcription.
    • It binds to the DNA template and facilitates the synthesis of RNA molecules.
    • RNA polymerase reads the DNA template in a specific direction (from 3' to 5') and synthesizes the RNA molecule accordingly.
  3. RNA Nucleotides:

    • RNA nucleotides are the building blocks used to construct the
In eukaryotic cells, pre-mRNAs undergo three main processing steps:
  • Capping at the 5' end.
  • Addition of a polyA tail at the 3' end. and.
  • Splicing to remove introns.

What events occur during mRNA processing?

In eukaryotic cells, before RNA polymerase II-generated transcripts could be translated into protein products, these transcripts (pre-mRNAs) need to be suitably processed to form messenger RNA (mRNA). Three major events constitute pre-mRNA processing: (a) 5′-end capping, (b) splicing, and (c) 3′-end polyadenylation.

What are the two major functions of mRNA?

The role of mRNA is to carry protein information from the DNA in a cell's nucleus to the cell's cytoplasm (watery interior), where the protein-making machinery reads the mRNA sequence and translates each three-base codon into its corresponding amino acid in a growing protein chain.

What happens in mRNA modification?

The pre-mRNA has to go through some modifications to become a mature mRNA molecule that can leave the nucleus and be translated. These include splicing, capping, and addition of a poly-A tail, all of which can potentially be regulated – sped up, slowed down, or altered to result in a different product.

What are the two parts of mRNA?

MRNAs have three parts: Nucleotides at the 5′ end provide binding sites for proteins that initiate polypeptide synthesis; nucleotides in the middle specify the sequence of amino acids in the polypeptide; and nucleotides at the 3′ end regulate the stability of the mRNA (Fig. 1.1).

What is the template for RNA during transcription?

Transcription uses one of the two exposed DNA strands as a template; this strand is called the template strand. The RNA product is complementary to the template strand and is almost identical to the other DNA strand, called the nontemplate (or coding) strand.

What molecule is the template for RNA?

DNA template

RNA molecules originate from a DNA template, through the process of transcription. That is, a single strand of RNA is transcribed from one of the strands of a DNA helix. Namely, the DNA molecule unwinds at the site of the gene to be transcribed.

Frequently Asked Questions

What is the template for transcription quizlet?

According to the central dogma, double-stranded DNA serves as the template for the production of RNA during transcription.

Which protein catalyses the formation of the RNA strand?

Simultaneously, the RNA polymerase synthesizes a single strand of pre-mRNA in the 5'-to-3' direction by catalysing the formation of phosphodiester bonds between activated nucleotides (free in the nucleus) that are capable of complementary base pairing with the template strand.

What is the enzyme that catalyzes the construction of an mRNA molecule?

RNA polymerase II

During transcription, the DNA of a gene serves as a template for complementary base-pairing, and an enzyme called RNA polymerase II catalyzes the formation of a pre-mRNA molecule, which is then processed to form mature mRNA (Figure 1).

What are the three major processing steps that occur in the maturation of mRNA?

In eukaryotic cells, pre-mRNAs undergo three main processing steps:
  • Capping at the 5' end.
  • Addition of a poly(A) tail at the 3' end.
  • Splicing to remove introns.


What are the stages of the mRNA?

Translation is generally divided into three stages: initiation, elongation, and termination (Figure 7.8). In both prokaryotes and eukaryotes the first step of the initiation stage is the binding of a specific initiator methionyl tRNA and the mRNA to the small ribosomal subunit.

What is used in transription as a template for the construction of a new rna molecule

... RNA. In transcription, _____ is used as a template for the construction of a new RNA molecule. a segment of one strand of doublestranded DNA. The genetic code 

What is the formation of protein from RNA called?
Translation: The process of synthesizing a protein from RNA is known as translation. The ribosome decodes messenger RNA (mRNA), which is created by transcription, to make a specific amino acid chain, or polypeptide, which will subsequently fold into an active protein.

What type of catalyst is made of protein or RNA?


An enzyme is a biological catalyst that is usually a protein but could be RNA. The point of a catalyst is to increase the speed with which a reaction happens.

What is used in transcription as a template for the construction of a new rna molecule

What is RNA made of protein?

RNA makes proteins using amino acids. There are 20 different types of amino acids that make up a protein's primary structure. Once a ribosome binds to an mRNA transcript, it starts decoding the mRNA codons and recruits tRNAs with the encoded amino acid. Codons are deciphered using the genetic code.

What is a catalyst composed of RNA called?

Ribozymes (ribonucleic acid enzymes) are RNA molecules that have the ability to catalyze specific biochemical reactions, including RNA splicing in gene expression, similar to the action of protein enzymes.

What catalyzes the transcription of mRNA?

The enzymes that perform transcription are called RNA polymerases. Like the DNA polymerase that catalyzes DNA replication (discussed in Chapter 5), RNA polymerases catalyze the formation of the phosphodiester bonds that link the nucleotides together to form a linear chain.

Which enzyme catalyzes the formation of mRNA quizlet?

The enzyme RNA polymerase catalyzes transcription of DNA, also known as mRNA synthesis (or production).

  • What enzymes catalyze translation?
    • Translation is catalyzed by a large enzyme called a ribosome, which contains proteins and ribosomal RNA (rRNA). Translation also involves specific RNA molecules called transfer RNA (t-RNA) which can bind to three basepair codons on a messenger RNA (mRNA) and also carry the appropriate amino acid encoded by the codon.

  • What is the enzyme that builds mRNA from DNA quizlet?
    • RNA polymerase is the enzyme that builds mRNA on the template of DNA. The enzyme initially begins where there is a promoter sequence in the DNA. Transcription factors mediate the binding of RNA polymerase and the initiation of transcription.

  • Which enzyme catalyzes the formation of DNA over RNA?
    • The enzyme catalyses the synthesis of DNA from RNA in retroviruses is reverse polymerase.

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